Business U.S. Federal Route 27 runs north-south in Nicholasville. It runs along a former alignment of U.S. Rt. 27 through the central part of the city. BUSN U.S. Rt. 27 passes through the following town in Jessamine County. Nicholasville
BUSN U.S. Rt. 27 is multi-plexed in Jessamine County. Kentucky State Route 169, at Nicholasville. BUSN U.S. Rt. 27 intersects the following highways in Jessamine County. U.S. Rt. 27, at Nicholasville.
A junction marker on SB U.S. Rt. 27. SB BUSN U.S. Rt. 27 north of KY St Rt 169. SB BUSN U.S. Rt. 27 south of KY St Rt 169. SB BUSN U.S. Rt. 27 south of Nicholasville.
