Wisconsin State Route 35 is an east-west highway in Southern Trempealeau County. It runs from the Buffalo/Trempealeau County Line, near Marshland, to the Trempealeau/La Crosse County Line, near New Amsterdam. WI St Rt 35 passes through the following towns in Trempealeau County. Centerville
Trempealeau WI St Rt 35 is multi-plexed in Trempealeau County. Wisconsin State Route 54, from the Buffalo/Trempealeau County Line, to Centerville.