U.S. Federal Route 53 is a north-south highway in Northwestern La Crosse County. It runs from a junction with U.S. Rt. 14/61; Wisconsin State Route 16/33 in La Crosse to the La Crosse/Trempealeau County Line, near Galesville. U.S. Rt. 53 passes through the following towns in La Crosse County. La Crosse Onalaska
A junction marker on WB U.S. Rt. 14/NB U.S. Rt. 61. A junction marker on WB WI St Rt 16. A BGS on WB I-90/SB U.S. Rt. 53. A BGS on WB I-90. SB I-90/EB WI St Rt 157 at I-90. SB I-90/EB WI St Rt 157 north of I-90. A BGS on SB U.S. Rt. 53/EB WI St Rt 157. SB U.S. Rt. 53/EB WI St Rt 157 north of I-90. SB U.S. Rt. 53 north of WI St Rt 157. SB U.S. Rt. 53/WI St Rt 35 north of LCCH MH. SB U.S. Rt. 53/WI St Rt 35 south of WI St Rt 93. SB U.S. Rt. 53/WI St Rt 93 north of WI St Rt 35. SB U.S Rt 53/WI St Rt 93 south of LCCH T. SB U.S. Rt. 53/WI St Rt 93 near Council Bay. SB U.S. Rt. 53/WI St Rt 93 at the La Crosse/Trempealeau County Line.