U.S. Federal Route 53 is a north-south highway in Eastern Trempealeau County. It runs from Eau Claire/Trempealeau County Line, Osseo, to the La Crosse/Trempealeau County Line, near Council Bay. U.S. Rt. 53 passes through the following towns in Trempealeau County. Osseo Pigeon Falls Coral City Whitehall Blair Beach Corner Frenchville Galesville
NB U.S. Rt. 53 north of WI St Rt 54/93. A junction marker on EB WI St Rt 54/SB WI St Rt 93. SB U.S. Rt. 53/WI St Rt 93; EB WI St Rt 54. SB U.S. Rt. 53/WI St Rt 93 south of east WI St Rt 54.