U.S. Federal Route 301 runs north-south in Eastern Prince George's County. It goes from the Charles/Prince Geroge's County Line, near Mattawoman, to the Anne Arundel/Prince Geroge's County Line, near Bowie. This is a multi-lane divided highway through out the county. U.S. Rt. 301 passes through the following towns in Prince George's County. Cheltenham Rosaryville Bowie U.S. Rt. 301 is multi-plexed in Prince George's County. Maryland State Route 5, from the Charles/Prince George's County Line, near Mattawoman to near Brandywine. Interstate 595, near Bowie. U.S. Rt. 50, near Bowie. U.S. Rt. 301 intersects the following highways in Prince George's County. Maryland State Route 5, near Brandywine. Maryland State Route 381, near Brandywine. Maryland State Route 382, near Mellwood. Maryland State Route 4 near Upper Marlboro. Maryland State Route 724, near Upper Marlboro. Maryland State Route 214, near Bowie. Maryland State Route 197, at Bowie. Interstate 595, at Bowie. U.S. Rt. 50, at Bowie.
WB I-595/U.S. Rt. 50; SB U.S. Rt. 301 at the Anne Arundel/Prince George's County Line. A BGS on WB I-595/U.S. Rt. 50; SB U.S. Rt. 301. A BGS on WB I-595/U.S. Rt. 50; SB U.S. Rt. 301. A BGS on WB I-595/U.S. Rt. 50; SB U.S. Rt. 301. A guide sign on SB MD St Rt 3.
