U.S. Federal Route 54 is the main north-south highway in Callaway County. It runs the length of the county. This goes from the Callaway/Cole County Line, at Jefferson City, to the Audrain/Callaway County Line, near Auxvasse. U.S. Rt. 54 passes through the following towns in Callaway County. Jefferson City
Lake Mykee New Bloomfield
Auxvasse U.S. Rt. 54 is multi-plexed in Callaway County. U.S. Rt. 63, at Jefferson City.